CIE Biology Paper-4: Specimen Questions 1 - 2 of 120

Question number: 1

One Liner Question▾

Write in Brief

What four steps are mainly used in gene cloning?

Explanation

Gene cloning is also called as molecular cloning which is used to assemble recombinant DNA molecule and their direct replication in the host organism.

Major steps, which are used for gene cloning are-

shows the given steps

select this step will be sequence

1. Generation of DNA Fragments→2. Joining to a vector→ 3. Introduction into a host cell for amplification →4. Selection of required sequence

Image shows cloning of DNA fragments

Image shows cloning of DNA fragments

Image shows cloning of DNA fragments in first step DNA is isolated and divided into separate parts in second step the fragments of DNA are join to the carrier molecule then recombinant DNA molecules introduced into a host cell.

Question number: 2

One Liner Question▾

Write in Brief

Diagram shows a cross section of plant cell. Fill the all organelles in given diagram. In addition, write their functions.

Diagram shows a cross section of plant cell

Diagram shows a cross section of plant cell

Diagram shows a cross section of plant cell with its organelles in it identify names of organelles

Explanation

shows a cross section of plant cell

shows a cross section of plant cell

Diagram shows a cross section of plant cell with its organelles

shows the cell organelle

shows table in cell organelle and Functions

Cell organelle

Functions

Cell wall

Support and protection

Cell membrane

Support, protection and controls movement of materials in/out of cell

Nucleus

Controls cell activities

Cytoplasm

Protects cell organelles

endoplasmic

reticulum

Carries materials through cell

ribosome

Produces proteins

Mitochondria

Breaks down sugar molecules into energy

Vacuole

Store food, water, waste

Lysosome

Digests old cell parts

chloroplast

Uses energy from sun to make food for the plant (photosynthesis)

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